Research Article | 11 Mar 2025

Comparative distribution and antibiotic susceptibility of extended-spectrum beta-lactamase- and non-extended-spectrum beta-lactamase-producing Proteus mirabilis in wound infections at Zainoel Abidin General Hospital, Banda Aceh, Indonesia

Suhartono Suhartono1, Zinatul Hayati2, Wilda Mahdani2, and Jufra Fonna1Show more

1. Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Syiah Kuala. Jl. Tgk. Syech Abdurrauf No. 3, Darussalam, Banda Aceh 23111, Aceh, Indonesia.

2. Department of Microbiology, Faculty of Medicine, Universitas Syiah Kuala. Jl. T. Tanoeh Abe, Darussalam, Banda Aceh 23111, Aceh, Indonesia.

Corresponding author: Suhartono Suhartono (suhartono@usk.ac.id)

Received: 2024-10-15, Accepted: 2025-02-07, Published: 2025-03-11

VETERINARY WORLD | pg no. 54-61 | Vol. 11, Issue 1 | DOI: 10.14202/vetworld.2025.54-61
Citations: —
View PDF

Cite this Article

  • APA
  • MLA
  • Chicago
  • Vancouver
  • Harvard

                
              

            

          

        


        



        
        

      

        
Abstract

          
Background and Aim: Proteus mirabilis, an opportunistic pathogen of the Enterobacteriaceae family, is frequently implicated in wound infections and exhibits significant antibiotic resistance, particularly through the production of extended-spectrum beta-lactamase (ESBL). This study aimed to determine the occurrence and antibiotic susceptibility of ESBL-producing and non-ESBL P. mirabilis isolates in wound infections at Zainoel Abidin General Hospital, Banda Aceh, Indonesia.
Materials and Methods: A cross-sectional study was conducted on wound specimens collected between January 2021 and March 2024. Bacterial identification and antimicrobial susceptibility testing were performed using the VITEK® 2 Compact system. Statistical analyses were conducted using Chi-square or Fisher statistical analyses significance set at p ≤ 0.05.
Results: A total of 153 P. mirabilis isolates were identified, of which 60 (39.22%) were ESBL-producing and 93 (60.78%) were non-ESBL-producing. The highest occurrence was observed in male patients (55% ESBL and 54.84% non-ESBL) and in patients older than 55 years (48.33% ESBL and 38.71% non-ESBL). Antibiotic susceptibility testing revealed that ESBL-producing isolates were highly susceptible to cefoperazone/sulbactam (98.67%), meropenem (98.33%), amikacin (96.67%), and piperacillin/tazobactam (91.67%). Non-ESBL isolates exhibited the highest susceptibility to amikacin (97.85%), cefoperazone/sulbactam (96.77%), piperacillin/tazobactam (91.67%), and ceftazidime (90.32%). Notably, ESBL-producing isolates exhibited resistance to amoxicillin (0%), ampicillin (1.67%), cefotaxime (8.33%), and levofloxacin (10%).
Conclusion: The high occurrence of ESBL-producing P. mirabilis, particularly in elderly patients, underscores the need for routine ESBL screening and targeted antibiotic therapy. The observed differences in antibiotic susceptibility between ESBL and non-ESBL isolates highlight the importance of early detection for appropriate antibiotic selection in wound infection management. Continued surveillance and antimicrobial stewardship are crucial in mitigating the impact of antibiotic-resistant P. mirabilis in clinical settings.

         
Keywords: antibiotic resistance, antimicrobial susceptibility, extended-spectrum beta-lactamase, Proteus mirabilis, wound infection.