Characterization of hemagglutination activity of emerging Newcastle disease virus in Bangladesh

Research Article

International Journal of One Health, 3: 28-35

https://doi.org/10.14202/IJOH.2017.28-35

Helal Uddin^1,2, Kamrul Islam^3,4,2, Mukti Barua⁵, Shariful Islam⁶, and Abdul Ahad²

1. Department of Livestock Services, Ministry of Fisheries and Livestock, Government of the People's Republic of Bangladesh, Bangladesh.
2. Department of Microbiology and Veterinary Public Health, Faculty of Veterinary Medicine, Chittagong Veterinary and Animal Sciences University, Chittagong, Bangladesh.
3. Biosafety & Biosecurity Consultant, One Health Epidemiology Fellow, Institute of Epidemiology, Disease Control and Research (IEDCR), Dhaka, Bangladesh.
4. Institute of Veterinary, Animal and Biomedical Sciences, Massey University, New Zealand.
5. Department of Animal Science and Nutrition, Chittagong Veterinary and Animal Sciences University, Chittagong, Bangladesh.
6. EcoHealth Alliance, New York, USA.

Background and Aim: Newcastle disease (ND) is an important viral disease for poultry caused by avian paramyxovirus which can be identified by its nature of agglutination activity with red blood cell (RBC) of different species. The study was aimed to characterize the hemagglutinating (HA) activity of ND virus (NDV) at three different temperatures using RBC of five avian species, six mammalian species, and eight different human blood groups.

Materials and Methods: The study was conducted from January to December 2014 at Chittagong Veterinary and Animal Sciences University. Five avian and six different mammalian species were selected for the study. In each species, two blood samples were collected aseptically. Eight different blood groups (A+, A-, B+, B-, AB+, AB-, O+, and O-) were studied in human. HA test was performed using two virus strains ND lasota and field isolate of very virulent NDV (VVNDV) with mentioned species of RBC at chilling (4℃), incubating (37℃), and room temperature (24℃).

Results: Avian RBC requires less time for agglutination than mammalian RBC. Incubation temperature (37℃) requires lowest time and chilling temperature requires highest time for agglutination of RBC. Duck RBC requires lowest time (17.81 min) while chicken RBC needs highest (57.5 min) time for HA at incubation temperature and at chilling temperature, respectively, against ND lasota virus and with field strain. Goat RBC requires significantly higher time for HA (184.68 min) at chilling temperature than other mammalian species. Human RBC requires almost similar time but O+ and O- blood group do not show any HA activity. Significant variation (p<0.05) found in quail RBC at incubation temperature. In mammalian species, a significant difference (p<0.05) has been observed in goat and horse RBC at chilling; horse and dog RBC at incubation; goat, horse, buffalo, and dog RBC at room temperature. In human, significant variation (p<0.05) has been found in A+, A- and B- blood group in chilling, in B+ blood group at incubation and A+, B+, B-, AB- blood group at room temperature against two virus strains.

Conclusion: ND is considered as an economically significant disease which is highly contagious in nature infecting many avian species. The threat of ND outbreak to poultry industry necessitates effective control measures to reduce the burden in commercial and backyard farming in Bangladesh. Keywords: chilling temperature, hemagglutination, incubation temperature, Newcastle disease virus, Newcastle disease virus lasota strain, very virulent Newcastle disease virus strain.

Keywords: chilling temperature, hemagglutination, incubation temperature, Newcastle disease virus, Newcastle disease virus lasota strain, very virulent Newcastle disease virus strain.

How to cite this article: Uddin H, Islam K, Barua M, Islam S, Ahad A. Characterization of hemagglutination activity of emerging Newcastle disease virus in Bangladesh. Int J One Health 2017;3:28-35.

Received: 21-03-2017 Published online: 06-06-2017

Corresponding author: Kamrul Islam E-mail: kamruldvm13@gmail.com

DOI: 10.14202/IJOH.2017.28-35

Copyright: Uddin, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.