Comparison of rose bengal plate test, serum agglutination test, and indirect enzyme-linked immunosorbent assay in brucellosis detection for human and goat samples

Research Article

International Journal of One Health, 4: 35-39

https://doi.org/10.14202/IJOH.2018.35-39

Ipola Patrick Albert^1,2, Charles Drago Kato², Kokas Ikwap², Steven Kakooza^3,4, Benson Ngolobe², Dickson Ndoboli¹, and Gabriel Tumwine¹

1. Uganda Research Training Collaborative, Kampala, Uganda.
2. Department of Biomolecular Resources and Biolab Sciences College of Veterinary Medicine Animal Resources and Biosecurity, Makerere University, P. O. Box 7062 Kampala, Uganda.
3. Central Diagnostic Laboratory, College of Veterinary Medicine Animal Resources and Biosecurity, Makerere University, P. O. Box 7062 Kampala, Uganda.
4. Novel Minds Science Plexus, Uganda.

Background and Aim: This study aimed at estimating the degree of agreement, specificity and sensitivity of RBPT, SAT, and ELISA in brucellosis detection in human and goat samples.

Materials and Methods: An analytical study was conducted involving parallel testing of 425 goat samples and 177 human samples to compare RBPT, SAT, and i-ELISA tests of brucellosis detection.

Results: A seroprevalence of 14.82% in animals and 11.86% in humans was detected using RBPT, 7.06% in goats and 5.09% in humans using SAT, and 6.35% in humans and 4.52% in animals using i-ELISA. The degree of agreement between the three tests in humans and animals was compared using Fleiss' kappa statistics where a moderate agreement (MA) was obtained for both with kappa value 0.57 in animals and 0.51 in humans and p<0.001. Kappa values in animals were 0.52 (MA) for RBPT and i-ELISA, 0.61 (MA) for RBPT and SAT, and 0.82 substantial agreement (SA) for SAT and i-ELISA. The kappa values in humans were 0.52 (MA) for RBPT and i-ELISA, 0.57 (MA) for RBPT and SAT, and 0.82 (SA) for SAT and i-ELISA, respectively. The study revealed a significant agreement in the tests for all the three tests, but there was SA between SAT and i-ELISA in both animal and human samples. Sensitivity and specificity of RBT and SAT were estimated using i-ELISA as a gold standard as follows, in goats RBPT: 100 and 91% and SAT: 100 and 99%, respectively, and in humans RBPT: 100 and 92% and SAT: 88 and 99%, respectively.

Conclusion: It appears that RBPT is good as a screening test, whereas SAT and i-ELISA are good for diagnosis purposes or confirmatory test. Keywords: brucellosis, indirect enzyme-linked immunosorbent assay, rose bengal plate test, serum agglutination test, Uganda.

Keywords: brucellosis, indirect enzyme-linked immunosorbent assay, rose bengal plate test, serum agglutination test, Uganda.

How to cite this article: Ipola PA, Kato CD, Ikwap K, Kakooza S, Ngolobe B, Ndoboli D, Tumwine G. Comparison of rose bengal plate test, serum agglutination test, and indirect enzyme-linked immunosorbent assay in brucellosis detection for human and goat samples.Int J One Health 2018;4:35-39.

Received: 23-02-2018 Accepted: 18-06-2018 Published online: 11-07-2018

Corresponding author: Ipola Patrick Albert E-mail: patrickalbert4@outlook.com

DOI: 10.14202/IJOH.2018.35-39

Copyright: Ipola, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.