Article history: Received: 01-02-2018, Accepted: 07-03-2018, Published online: 03-04-2018
Corresponding author: Ekene Vivienne Ezenduka
E-mail: email@example.comCitation: Ezenduka EV, Okonkwo BC, Anyaoha CO, Nwanta JA, Anaga A. Immunologic determination of chloramphenicol residue in commercial birds at Nsukka, Enugu State, Southeast Nigeria. Int J One Health 2018;4:22-27.
Aim: This study aimed to determine the presence and prevalence of chloramphenicol (CAP, a drug which was banned for use in food-producing animals due to many side effects) residue in commercial birds slaughtered at Ikpa abattoir and its awareness and usage in farms at Nsukka, Enugu State, Nigeria.
Materials and Methods: A cross-sectional survey was done with the use of a questionnaire on usage and awareness of CAP and screening for its presence in commercial poultry in the study area. The questionnaire was supplied to 35 commercial farms, and liver samples from 300 commercial broilers were analyzed using an enzyme-linked immunosorbent assay technique; the prevalence was then determined.
Results: Of the 35 farms evaluated, 33 (94%) responded. In the management practice, 57.6% of the farms use intensive deep litter, 18.2% intensive battery cage, and 24.2% extensive farming system. 19 (69.7%) farms rear only broilers, 12.1% layers, and 15.1% both. The feeding management showed that 21.1% of farmers produce their own feed with inclusion of antibiotics while 78.8% use commercial feed, of which 11.5% incorporate antibiotics. The findings also showed that 54.4% of the respondents use CAP and only 30.3% are aware of the consequences of antimicrobial residue in food and have knowledge of the legislation on the prudent use of antimicrobials in food animals. Of the 300 samples screened for CAP residue, 18.7% were positive with concentrations ranging from 0.5 to 6.2 parts per billion.
Conclusion: CAP is still very much in use in the study area, despite the ban, and it is present in the tissues of commercial birds meant for human consumption.
Keywords: chloramphenicol, drug residue, enzyme-linked immunosorbent assay, liver, poultry.
1. Anadon A, Bringas P, Lorronga MR, Dial MJ. Bioavailability, pharmacokinetics and residues of chloramphenicol in chicken. J Vet Pharmacol Therapeut 1994;17:52-8. [Crossref]
3. Kan CA, Petz M. Residues of veterinary drugs in eggs and their distribution between yolk and white. J Agric Food Chem 2000;48:6397-403. [Crossref]
4. Haihong H, Guyue C, Zahid I, Xiaohui A, Hafiz IH, Lingli H, et al. Benefits and risks of antimicrobial use in food-producing animals. Front Microbiol 2014;5:288.
5. Bane DP, Kniffen TS, Hall WF. Sulfamethazine residue in swine: Comparison of on farm monitoring methods. Prev Med 1989;7:303-9. [Crossref]
6. Mauricio S, Chris K, Rohan . The danger of chloramphenicol in milk. Food Saf Mag 2013. Available from: https://www.foodsafetymagazine.com/signature-series/the-danger-of-chloramphenicol-in-milk. [Last accessed on 2017 Dec 17].
7. Papich MG. Precautionary Information on Adverse Reactions and Side Effects of Chloramphenicol in Saunders Handbook of Veterinary Drugs. 4th ed. St. Louis, MO: Saunders; 2016. p. 148-50.
8. FDA: FDA Takes Significant Steps to Address Antimicrobial Resistance; 2013, December 11. Available from: https//www.fda.gov/ForConsumers/ConsumerUpdates/ucm378100.htm. [Last assessed on 2016 Mar 06].
9. US Food and Drug Administration Chloromphenical as an Unapproved New Animal Drug-Direct Reference Seizure Authority-CPG Sec 645.300; 2014. Available from: https://www.fda.gov/ICECI/ComplianceManuals/CompliancePolicyGuidanceManual/ucm074681.htm. [Last assessed on 2016 Mar 10].
10. Mbodi F, Nguku P, Okolocha E, Kabir J. Determination of chloramphenicol residues in commercial chicken eggs in the federal capital territory (FCA), Abuja, Nigeria. Food Addit Contam Part A 2014;31:1834-9. [Crossref] [PubMed]
11. Settepani JA. The hazard of using chloramphenicol in food animals. J Am Vet Med Assoc 1984;184:930-1. [PubMed]
12. WHO. Evaluation of Certain Veterinary Drug Residues in Food. 32nd Report on the Joint FAO/WHO Expert. Committee on Food Additives WHO Technical Report Series No. 763. Geneva: WHO; 1988.
13. Wongtavatcha J, Mclean JG, Romos F, Arnold D. Chloramphenicol. Geneva: World Health Organization Food Additives Series 53; 2004.
14. EFSA Panel on Contaminants in the Food Chain (CONTAM). Scientific opinion on chloramphenicol in food and feed. EFSA J 2014;12:3907.
15. Black LA. Aspects of the Pharmacokinetics and Pharmacodynamics of Chloramphenicol, Enrofloxacin and Fluconazole in Koalas (Phascolarctos cinereus). PhD Theses, University of Sydney, Faculty of Veterinary Science; 2013. Available from: http://www.hdl.handle.net/2123/11597. Accesed on 06-03-2016.
16. Regulatory Control of Veterinary Drug. Department of Primary Industry Pesticides Section Document PB 237 A. Canberra: Australian Government Publishing Service; 1983.
17. AL-Ghamdi MS, AL-Mustafa ZH, EL Morsy F, AL-Faky A, Haider I, Essa H. Residues of tetracycline compounds in poultry products in the eastern province of Saudi Arabia. Public Health 2000;114:300-4. [Crossref]
18. Ezenduka EV, Oboegbulem SI, Nwanta JA. Rapid detection of antimicrobial residues in poultry: A consequence of non-prudent use of antimicrobials. Health 2014;6:1-4. [Crossref]
19. Ezenduka EV, Oboegbulem SI, Nwanta JA, Onunkwo JI. Prevalence of antimicrobial residues in raw table eggs from farms and retail outlets in Enugu state, Nigeria. Trop Anim Health Prod 2011;43:557-9. [Crossref] [PubMed]
20. Kabiri JU, Umoh VJ, Okoh US, Kwaga JK. Veterinary drug use in poultry farms and determination of antimicrobial drug residues in commercial eggs and slaughtered chicken in Kaduna State, Nigeria. Elsevier Science Ltd. Food Control 2003;15:99-105.
21. Olatoye IO, Oyelakin E, Adeyemi IG, Call DR. Chloramphenicol use and prevalence of its residues in broiler chickens and eggs in Ibadan, Nigeria. Niger Vet J 2012;33:643-10.
22. Omeiza GK, Kabir J, Mamman M, Ibrahim H, Fagbamila IO. Response of Nigerian farmers to a questionnaire on chloramphenical application in commercial layers. Vet Ital 2012;48:88-93.
23. Nonga HE, Simon C, Karimuribo ED, Mdegela RH. Assessment of antimicrobial usage and residues in commercial chicken eggs from smallholder poultry keepers in Morogoro municipality, Tanzania. Zoonoses Public Health 2010;57:339-44. [PubMed]
24. Cannavan A. Capacity Building for Veterinary Drug Monitoring Programs in Developing Countries. In: FAO/WHO Technical Workshop on Residues of Veterinary Drugs without ADI/MRL; 2004. Available from: http://www.fao.org/docrep/008. Last assessed on 06-03-2016.