International Journal of One Health

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Research (Published online: 16-01-2018)

1. A cross-sectional study on prevalence of bovine tuberculosis in Indian and crossbred cattle in Gangetic delta region of West Bengal, India - Ratan Das, Premanshu Dandapat, Arijit Chakrabarty, Pramod Kumar Nanda, Samiran Bandyopadhyay and Subhasish Bandyopadhyay

International Journal of One Health, 4: 1-7

 

 

  doi: 10.14202/IJOH.2018.1-7

 

Ratan Das: District Veterinary Hospital, Animal Resources Development Department, Government of Tripura, Ambassa, Dhalai, Tripura, India.

Premanshu Dandapat: Eastern Regional Station, ICAR-Indian Veterinary Research Institute, Kolkata - 700 037, West Bengal, India.

Arijit Chakrabarty: Eastern Regional Station, ICAR-Indian Veterinary Research Institute, Kolkata - 700 037, West Bengal, India.

Pramod Kumar Nanda: Eastern Regional Station, ICAR-Indian Veterinary Research Institute, Kolkata - 700 037, West Bengal, India.

Samiran Bandyopadhyay: Eastern Regional Station, ICAR-Indian Veterinary Research Institute, Kolkata - 700 037, West Bengal, India.

Subhasish Bandyopadhyay: Eastern Regional Station, ICAR-Indian Veterinary Research Institute, Kolkata - 700 037, West Bengal, India.

 

Received: 23-09-2017, Accepted: 18-12-2017, Published online: 16-01-2018

 

Corresponding author: Premanshu Dandapat, e-mail: pdandapat@gmail.com


Citation: Das R, Dandapat P, Chakrabarty A, Nanda PK, Bandyopadhyay S, Bandyopadhyay S. A cross-sectional study on prevalence of bovine tuberculosis in Indian and crossbred cattle in Gangetic delta region of West Bengal, India. Int J One Health 2018;4:1-7.


Abstract


Aim: The aim of this study was to investigate the incidence of bovine tuberculosis (BTB), an old chronic disease having zoonotic potential, covering four districts in Gangetic delta region of West Bengal, India, and to find the prevalence in organized as well as backyard herds and variation in relation to their age, sex, and breeds.

Methods: The incidence of BTB in exotic and indigenous breeds of cattle (n=173) of various age groups was investigated employing tuberculin (single intradermal tuberculin and comparative cervical tuberculin) tests and gamma interferon assay. Further, milk samples (n=96) from milching animals and antemortem (n=519) samples (nasal swab, buccal swab, and aspirates from pre-scapular lymph nodes) were also screened employing bacteriological and molecular techniques.

Results: In total, 36 (25.4%) animals from organized and one (3.2%) from backyard farming sector were found positive to BTB. Polymerase chain reaction (PCR) of milk samples based on 16S rRNA amplified the 1030 bp band in four samples indicating them belonging to genus Mycobacterium. Species-specific primers used to differentiate between Mycobacterium bovis and M. tuberculosis confirmed the presence of M. bovis. Prevalence of BTB in exotic crossbred animals (34.6%) was significantly higher (p<0.001) compared to indigenous cattle (10.5%). Further, gender-wise analysis of data with respect to BTB revealed higher positivity (p<0.05) among cows/heifers (25.8%) compared to bulls/bullocks (7.3%). Although BTB-positive cattle were detected in all the age groups, no statistical difference (p=0.779) was found among them.

Conclusion: The findings indicate a higher prevalence of BTB in exotic crossbred animals in Gangetic delta and variation in breed susceptibility, thereby suggesting an urgent review of the present policy on adopting national crossbreeding program and implementation of “One Health” approach.

Keywords: bovine tuberculosis, cattle, India, prevalence, West Bengal.


References


1. Ameni G, Aseffa A, Engers H, Young D, Gordon S, Hewinson G, et al. High prevalence and increased severity of pathology of bovine tuberculosis in Holsteins compared to zebu breeds under field cattle husbandry in central Ethiopia. Clin Vaccine Immunol 2007;14:1356-61.
https://doi.org/10.1128/CVI.00205-07
PMid:17761523 PMCid:PMC2168113
 
2. Le Roex N, Koets AP, Van Helden PD, Hoal EG. Gene polymorphisms in African buffalo associated with susceptibility to bovine tuberculosis infection. PLoS One 2013;8:E64494.
https://doi.org/10.1371/journal.pone.0064494
PMid:23691232 PMCid:PMC3654904
 
3. Malama S, Muma JB, Godfroid J. A review of tuberculosis at the wildlife-livestock-human interface in Zambia. Infect Dis Poverty 2013;2:13.
https://doi.org/10.1186/2049-9957-2-13
PMid:23849550 PMCid:PMC3734204
 
4. Zoonotic TB. World Health Organization; 2016. Available from: http://www.who.int/tb/zoonoticTB.pdf?ua=1. Last accessed on 12.09.2017
 
5. O'Reilly LM, Daborn CJ. The epidemiology of Mycobacterium bovis infections in animals and man: A review. Tuber Lung Dis 1995;76 Suppl 1:1-46.
https://doi.org/10.1016/0962-8479(95)90591-X
 
6. Cosivi O, Meslin FX, Daborn CJ, Grange JM. Epidemiology of Mycobacterium bovis infection in animals and humans, with particular reference to Africa. Rev Sci Tech 1995;14:733-46.
https://doi.org/10.20506/rst.14.3.875
PMid:8593405
 
7. Bezos J, Alvarez J, Romero B, De Juan L, Dominguez L. Bovine tuberculosis: Historical perspective. Res Vet Sci 2014;97:S3-4.
https://doi.org/10.1016/j.rvsc.2014.09.003
PMid:25278140
 
8. Schiller I, Oesch B, Vordermeier HM, Palmer MV, Harris BN, Orloski KA, et al. Bovine tuberculosis: A review of current and emerging diagnostic techniques in view of their relevance for disease control and eradication. Transbound Emerg Dis 2010;57:205-20.
https://doi.org/10.1111/j.1865-1682.2010.01148.x
 
9. OIE. Manual of Standards for Diagnostic Tests and Vaccines. Bovine Tuberc. Paris: OIE; 2008. p. 683-97.
 
10. Wood PR, Jones SL, Bovigam TM: An in vitro cellular diagnostic test for bovine tuberculosis. Tuberculosis 2001;81:147-55.
https://doi.org/10.1054/tube.2000.0272
PMid:11463236
 
11. Álvarez J, Perez A, Marqués S, Bezos J, Grau A, de la Cruz ML, et al. Risk factors associated with negative in-vivo diagnostic results in bovine tuberculosis-infected cattle in Spain. BMC Vet Res 2014;10:14.
https://doi.org/10.1186/1746-6148-10-14
PMid:24410926 PMCid:PMC3895706
 
12. Krasnow I, Wayne LG. Comparison of methods for tuberculosis bacteriology. Appl Microbiol 1969;18:915-7.
PMid:4984206 PMCid:PMC378113
 
13. De Lisle GW, Havill PF. Mycobacteria isolated from deer in New Zealand from 1970-1983. N Z Vet J 1985;33:138-40.
https://doi.org/10.1080/00480169.1985.35198
PMid:16031192
 
14. Baron EJ, Finegold JS. Mycobacteria and other bacteria with unusual growth requirements. In: Bailey and Scott's Diagnostic Microbiology. 10th ed. St. Louis: CV Mosby Co.; 2008. p. 714.
 
15. Wilton S, Cousins D. Detection and identification of multiple mycobacterial pathogens by DNA amplification in a single tube. PCR Methods Appl 1992;1:269-73.
https://doi.org/10.1101/gr.1.4.269
PMid:1282431
 
16. Das S, Das SC, Verma R. Occurrence of RD9 region and 500 bp fragment among clinical isolates of Mycobacterium tuberculosis and Mycobacterium bovis. Microbiol Immunol 2007;51:231-4.
https://doi.org/10.1111/j.1348-0421.2007.tb03905.x
PMid:17310091
 
17. Mukherjee F. Comparative prevalence of tuberculosis in two dairy herds in India. Rev Sci Tech 2006;25:1125-30.
https://doi.org/10.20506/rst.25.3.1717
PMid:17361775
 
18. Thakur A, Sharma M, Katoch VC, Dhar P, Katoch RC. A study on the prevalence of bovine tuberculosis in farmed dairy cattle in Himachal Pradesh. Vet World 2010;3:409-14.
https://doi.org/10.5455/vetworld.2010.408-413
 
19. Dhinakaran M, Rao VN, Nedunchellian S. Immunological Response in Tuberculin Reactor Cattle. In: Proceedings of the National Symposium on Recent Advances in Control of Diseases of Crossbred and Companion Animals. Mumbai, India: Konkan Krishi Vidyapeeth. Bombay Veterinary College, Department of Medicine; 1991.
 
20. Ameni G, Vordermeier M, Firdessa R, Aseffa A, Hewinson G, Gordon SV, et al. Mycobacterium tuberculosis infection in grazing cattle in central Ethiopia. Vet J 2011;188:359-61.
https://doi.org/10.1016/j.tvjl.2010.05.005
PMid:20965132 PMCid:PMC3103825
 
21. Coad M, Clifford DJ, Vordermeier HM, Whelan AO. The consequences of vaccination with the Johne's disease vaccine, Gudair, on diagnosis of bovine tuberculosis. Vet Rec 2013;172:266.
https://doi.org/10.1136/vr.101201
PMid:23475045
 
22. Aranaz A, De Juan L, Bezos J, Alvarez J, Romero B, Lozano F, et al. Assessment of diagnostic tools for eradication of bovine tuberculosis in cattle co-infected with Mycobacterium bovis and M. avium subsp. paratuberculosis. Vet Res 2006;37:593-606.
https://doi.org/10.1051/vetres:2006021
PMid:16701065
 
23. Monaghan ML, Doherty ML, Collins JD, Kazda JF, Quinn PJ. The tuberculin test. Vet Microbiol 1994;40:111-24.
https://doi.org/10.1016/0378-1135(94)90050-7
 
24. Bhutediya JM, Dandapat P, Chakrabarty A, Das R, Nanda PK, Bandyopadhyay S, et al. Prevalence of paratuberculosis in organized and unorganized dairy cattle herds in West Bengal, India. Vet World 2017;10:574-9.
https://doi.org/10.14202/vetworld.2017.574-579
PMid:28717306 PMCid:PMC5499071
 
25. Wood PR, Corner LA, Rothel JS, Baldock C, Jones SL, Cousins DB, et al. Field comparison of the interferon‐gamma assay and the intradermal tuberculin test for the diagnosis of bovine tuberculosis. Aust Vet J 1991;68:286-90.
https://doi.org/10.1111/j.1751-0813.1991.tb03254.x
PMid:1953560
 
26. Cagiola M, Feliziani F, Severi G, Pasquali P, Rutili D. Analysis of possible factors affecting the specificity of the gamma interferon test in tuberculosis-free cattle herds. Clin Diagn Lab Immunol 2004;11:952-6.
https://doi.org/10.1128/CDLI.11.5.952-956.2004
 
27. Srivastava K, Chauhan DS, Gupta P, Singh HB, Sharma VD, Yadav V, et al. Isolation of Mycobacterium bovis and M. tuberculosis from cattle of some farms in north India-possible relevance in human health. Indian J Med Res 2008;28:26.
 
28. Parsons LM, Brosch R, Cole ST, Somoskövi A, Loder A, Bretzel G, et al. Rapid and simple approach for identification of Mycobacterium tuberculosis complex isolates by PCR-based genomic deletion analysis. J Clin Microbiol 2002;40:2339-45.
https://doi.org/10.1128/JCM.40.7.2339-2345.2002
PMid:12089245 PMCid:PMC120548
 
29. Rodriguez JG, Mejia GA, Del Portillo P, Patarroyo ME, Murillo LA. Species-specific identification of Mycobacterium bovis by PCR. Microbiology 1995;141:2031-8.
https://doi.org/10.1099/13500872-141-9-2131
PMid:7496524
 
30. Sharma AK, Vanamayya PR, Parihar NS. Tuberculosis in cattle: A retrospective study based on necropsy. Indian J Vet Pathol 1985;9:14-8.
 
31. Brotherstone S, White IM, Coffey M, Downs SH, Mitchell AP, Clifton-Hadley RS, et al. Evidence of genetic resistance of cattle to infection with Mycobacterium bovis. J Dairy Sci 2010;93:1234-42.
https://doi.org/10.3168/jds.2009-2609
PMid:20172243
 
32. Dinka H, Duressa A. Prevalence of bovine tuberculosis in Arsi Zones of Oromia, Ethiopia. Afr J Agric Res 2011;6:3853-8.
 
33. Brooks-Pollock E, Conlan AJ, Mitchell AP, Blackwell R, McKinley TJ, Wood JL, et al. Age-dependent patterns of bovine tuberculosis in cattle. Vet Res 2013;44:97.
https://doi.org/10.1186/1297-9716-44-97
PMid:24131703 PMCid:PMC3853322
 
34. Moiane I, Machado A, Santos N, Nhambir A, Inlamea O, Hattendorf J, et al. Prevalence of bovine tuberculosis and risk factor assessment in cattle in rural livestock areas of Govuro district in the southeast of Mozambique. PLoS One 2014;9:e91527.
https://doi.org/10.1371/journal.pone.0091527
PMid:24632593 PMCid:PMC3954769
 

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